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olig1 antibody  (Millipore)


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    Structured Review

    Millipore olig1 antibody
    Olig1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/olig1+antibody/olig1+antibody/pmc11715087-590-0-1
    Average 90 stars, based on 1 article reviews
    olig1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Purification:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Control:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Western Blot:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Quantitative RT-PCR:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Incubation:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Expressing:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Immunofluorescence:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Staining:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).

    Clone Assay:

    Article Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum.
    Article Snippet: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis.. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination.. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells.


    Article Title: Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
    Article Snippet: Cells were incubated overnight with the following primary antibodies: nestin (Santa Cruz), musashi (Life Technologies), microtubule-associated protein 2 (Map2 (Millipore)), neurofilament light chain NF-l (Abcam), Olig1 and Olig2 (both from Millipore), myelin basic protein (MBP (Abcam)), and β -actin (Cell Signalling).

    Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells
    Article Snippet: The following primary antibodies were used: MAP2 (Abcam,; mouse immunoglobulin G 1 (IgG1), 1:1,000), GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), O4 (Millipore; mouse IgM, 1:100 in 1 PBS; incubated with cells at room temperature for 30 minutes before fixation), Sox2 (Millipore; rabbit polyclonal, 1:500), Nestin (BD biosciences; mouse IgG1, 1:500), Ki67 (Chemicon; mouse IgG1, 1:500), phospho-Smad1/5/8 (Cell Signaling; rabbit polyclonal, 1:500), ID2 (Santa Cruz; rabbit polyclonal, 1:100), ID4 (Santa Cruz; rabbit polyclonal, 1:50), OLIG1 (Millipore; mouse IgG2b, 1:250), OLIG2 (Millipore; mouse IgG2a, 1:250).

    Article Title: Olig1 expression pattern in neural cells during rat spinal cord development
    Article Snippet: The filters were blocked with 5% nonfat dry milk in TBS for 1 hour at RT and then incubated overnight at 4°C with primary antibodies (in TBST-5% bovine serum albumin [BSA]) including Olig1 (1:200, Millipore) and β-actin (1:400, Sigma) as markers of differentiated neural cells.

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: Olig1 (Millipore, cat no. MAB344, RRID: AB_94860, 1:200) and Olig2 (Millipore, cat no. AB9610, RRID: AB_570666, 1:400) antibodies were used to evaluate the presence of oligodendrocyte progenitors in the WBOs.

    Article Title: The Divalent Metal Transporter 1 (DMT1) Is Required for Iron Uptake and Normal Development of Oligodendrocyte Progenitor Cells
    Article Snippet: The primary antibodies used in the present study were against the following: caspase-3 (mouse; 1:200; Cell Signaling Technology; RRID: AB_2341188 ); CC1 (mouse; 1:300; Calbiochem; RRID: AB_2057371 ); Ki67 (rabbit; 1:250; Abcam; RRID: AB_443209 ); Ki67 (mouse; 1:250; BD Biosciences; RRID: AB_396287 ); MBP (mouse; 1:1000; Covance; RRID: AB_510039 ); MOG (mouse; 1:200; Millipore; RRID: AB_1587278 ); Olig1 (mouse; 1:500; Millipore; RRID: AB_92198 ); Olig2 (mouse and rabbit; 1:500; Millipore; RRID: AB_570666 and RRID: AB_10807410 ); PLP (rat; 1:50; AA3-PLP/DM20; RRID: AB_2341144 ); and Sox2 (rabbit; 1:200; Millipore; RRID: AB_2286686 ).

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Samples were incubated in the primary antibodies diluted in Tris-carrageenan-triton solution (TCT) (Tris-HCl buffer pH 7.8, 0.7% λ-carrageenan (Sigma), and triton 0.5% X-100 (Sigma)) O.N. at RT using the primary antibodies against: Oct4 (Abcam, Cambridge, UK, 1:100), Sox2 (Abcam, 1:100), SSEA-1 (Developmental Studies Hybridoma Bank (DSHB), 1:10), E-Cadherin (BD Bioscience, 1:15), Brachyury (Abcam, 1:100), α-fetoprotein (Abcam, 1:100), MAP2 (Millipore, 1:500), Nestin (DSHB, 1:10), Vimentin (Abcam, 1:50), Musashi (Abcam, 1:200), Doublecortin (DCX) (Abcam, 1:200), Olig1 (Millipore, 1:200), glial fibrillary acidic protein (GFAP) (Abcam, 1:500), and 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (Abcam, 1:200).



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    Image Search Results


    Fig. 5. RNF220 regulates K63-linked polyubiquitination of Olig1 and Olig2. (A) Co-IP analyses of the interaction between endogenous RNF220 and Olig1 or Olig2 in forebrains with the indicated antibodies. IgG, immunoglobulin G. (B) Ubiquitination analyses of polyubiquitinated Olig1 and Olig2 in the forebrains of P7 RNF220-WT (n = 3) and RNF220-cKO (n = 3) mice, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls. (C) Analyses of ubiquitination levels of Olig1 and Olig2 in the forebrains of P60 RNF220-iWT (n = 3) and RNF220-icKO (n = 3) mice with tamoxifen treatment or not, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls without tamoxifen treatment. (D) Ubiquitination analyses of RNF220-mediated polyubiquitination of Olig1 and Olig2 in the presence of WT or indicated ubiquitin mutants in HEK293 cells. HA, hemagglutinin. (E) Ubiquitination analyses of RNF220-mediated polyubiquitination of Olig1 and Olig2 with purified proteins as indicated in vitro. (F) Schematic diagram showing all lysine sites in mouse Olig1 and Olig2 proteins and the lysine sites targeted by RNF220 are highlighted in red. (G) Ubiquitination analyses of RNF220-mediated polyubiquitination of WT or Olig1/2 mutants in HEK293 cells as indicated. IP, immunoprecipitation; WCL, whole-cell lysate; TAM, tamoxifen. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction in (D) and unpaired Student’s t test in (F). n.s. (not significant), P > 0.05; **P < 0.01.

    Journal: Science advances

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.

    doi: 10.1126/sciadv.adk3931

    Figure Lengend Snippet: Fig. 5. RNF220 regulates K63-linked polyubiquitination of Olig1 and Olig2. (A) Co-IP analyses of the interaction between endogenous RNF220 and Olig1 or Olig2 in forebrains with the indicated antibodies. IgG, immunoglobulin G. (B) Ubiquitination analyses of polyubiquitinated Olig1 and Olig2 in the forebrains of P7 RNF220-WT (n = 3) and RNF220-cKO (n = 3) mice, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls. (C) Analyses of ubiquitination levels of Olig1 and Olig2 in the forebrains of P60 RNF220-iWT (n = 3) and RNF220-icKO (n = 3) mice with tamoxifen treatment or not, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls without tamoxifen treatment. (D) Ubiquitination analyses of RNF220-mediated polyubiquitination of Olig1 and Olig2 in the presence of WT or indicated ubiquitin mutants in HEK293 cells. HA, hemagglutinin. (E) Ubiquitination analyses of RNF220-mediated polyubiquitination of Olig1 and Olig2 with purified proteins as indicated in vitro. (F) Schematic diagram showing all lysine sites in mouse Olig1 and Olig2 proteins and the lysine sites targeted by RNF220 are highlighted in red. (G) Ubiquitination analyses of RNF220-mediated polyubiquitination of WT or Olig1/2 mutants in HEK293 cells as indicated. IP, immunoprecipitation; WCL, whole-cell lysate; TAM, tamoxifen. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction in (D) and unpaired Student’s t test in (F). n.s. (not significant), P > 0.05; **P < 0.01.

    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Techniques: Co-Immunoprecipitation Assay, Ubiquitin Proteomics, Expressing, Purification, In Vitro, Immunoprecipitation, Control, MANN-WHITNEY

    Fig. 6. RNF220 maintains Olig1 and Olig2 proteins stabilization through regulating their ubiquitination. (A) WB analyses of protein expression of WT or Olig1/2 mutants when coexpressed with WT RNF220 or mutants lacking ubiquitin E3 ligase activity in HEK293 cells. (B) WB analyses of endogenous protein expression of RNF220, Olig1, and Olig2 in OPC and OL cells, isolated as in fig. S1A, from the brains of P7 RNF220-WT (n = 3), RNF220-cHet (n = 3), and RNF220-cKO (n = 3) mice, and bar graphs (mean ± SD) show normalized levels against respective protein expression in the WT controls. (C) WB analyses of endogenous protein expression of RNF220, Olig1, and Olig2 in isolated OPC from the brains of P60 RNF220-iWT (n = 3) and RNF220-icKO (n = 3) mice with tamoxifen treatment or not, and bar graphs (mean ± SD) show normalized levels against respective protein expression from the WT control without tamoxifen treatment. (D) Cycloheximide chase analyses of protein half-lives of endogenous Olig1 and Olig2 in MOPC cells overexpressing WT RNF220 or RNF220W539R mutant lacking E3 ligase activity, and broken line graphs (mean ± SD) show normalized levels against respective protein expression from the control without cycloheximide treatment. ΔR, ΔRING; CHX, cycloheximide. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction. n.s. (not significant), P > 0.05; *P < 0.05; **P < 0.01.

    Journal: Science advances

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.

    doi: 10.1126/sciadv.adk3931

    Figure Lengend Snippet: Fig. 6. RNF220 maintains Olig1 and Olig2 proteins stabilization through regulating their ubiquitination. (A) WB analyses of protein expression of WT or Olig1/2 mutants when coexpressed with WT RNF220 or mutants lacking ubiquitin E3 ligase activity in HEK293 cells. (B) WB analyses of endogenous protein expression of RNF220, Olig1, and Olig2 in OPC and OL cells, isolated as in fig. S1A, from the brains of P7 RNF220-WT (n = 3), RNF220-cHet (n = 3), and RNF220-cKO (n = 3) mice, and bar graphs (mean ± SD) show normalized levels against respective protein expression in the WT controls. (C) WB analyses of endogenous protein expression of RNF220, Olig1, and Olig2 in isolated OPC from the brains of P60 RNF220-iWT (n = 3) and RNF220-icKO (n = 3) mice with tamoxifen treatment or not, and bar graphs (mean ± SD) show normalized levels against respective protein expression from the WT control without tamoxifen treatment. (D) Cycloheximide chase analyses of protein half-lives of endogenous Olig1 and Olig2 in MOPC cells overexpressing WT RNF220 or RNF220W539R mutant lacking E3 ligase activity, and broken line graphs (mean ± SD) show normalized levels against respective protein expression from the control without cycloheximide treatment. ΔR, ΔRING; CHX, cycloheximide. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction. n.s. (not significant), P > 0.05; *P < 0.05; **P < 0.01.

    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Techniques: Ubiquitin Proteomics, Expressing, Activity Assay, Isolation, Control, Mutagenesis, MANN-WHITNEY

    Fig. 7. Leukodystrophy-related mutations impair RNF220 regulation on Olig protein stabilization, oligodendroglial differentiation, and myelination. (A) Ubiq- uitination analyses of polyubiquitinated Olig1 and Olig2 when coexpressed with WT RNF220 or leukodystrophy-related missense mutants, RNF220R363Q and RNF220R365Q, in HEK293 cells. (B) WB analyses of endogenous Olig1 and Olig2 expression in MOPC cells overexpressing WT RNF220 or leukodystrophy-related missense mutants, RN- F220R363Q and RNF220R365Q. (C) Representative T2-weighted mouse brain MRI scanning of P60 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. The arrows indicate the corpus callosum. (D) Representative images showing myelin staining (BG) in corpus callosum regions of P60 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. (E) Electron microscopy images of the corpus callosum transverse sections from P60 RNF220-RR (n = 4), RNF220-RQ (n = 4), and RNF220-QQ (n = 4) mice. Scale bars, 2 μm. Bar graphs (mean ± SD) show quantification of the number and percentage of myelinated axons, and scatterplots show g-ratio relative to axon diameter. (F) Immunofluorescence staining assays of BrdU and Sox10 in the corpus callosum regions of P3 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. Scale bars, 50 μm. (G) Bar graphs (mean ± SD) show the quantification of BrdU+Sox10+ cells, Ki67+PDGFRα+ cells on P3, and CC1+Sox10+ cells on P21. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction or unpaired Student’s t test. n.s. (not significant), P > 0.05; **P < 0.01.

    Journal: Science advances

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.

    doi: 10.1126/sciadv.adk3931

    Figure Lengend Snippet: Fig. 7. Leukodystrophy-related mutations impair RNF220 regulation on Olig protein stabilization, oligodendroglial differentiation, and myelination. (A) Ubiq- uitination analyses of polyubiquitinated Olig1 and Olig2 when coexpressed with WT RNF220 or leukodystrophy-related missense mutants, RNF220R363Q and RNF220R365Q, in HEK293 cells. (B) WB analyses of endogenous Olig1 and Olig2 expression in MOPC cells overexpressing WT RNF220 or leukodystrophy-related missense mutants, RN- F220R363Q and RNF220R365Q. (C) Representative T2-weighted mouse brain MRI scanning of P60 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. The arrows indicate the corpus callosum. (D) Representative images showing myelin staining (BG) in corpus callosum regions of P60 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. (E) Electron microscopy images of the corpus callosum transverse sections from P60 RNF220-RR (n = 4), RNF220-RQ (n = 4), and RNF220-QQ (n = 4) mice. Scale bars, 2 μm. Bar graphs (mean ± SD) show quantification of the number and percentage of myelinated axons, and scatterplots show g-ratio relative to axon diameter. (F) Immunofluorescence staining assays of BrdU and Sox10 in the corpus callosum regions of P3 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220-QQ (n = 3) mice. Scale bars, 50 μm. (G) Bar graphs (mean ± SD) show the quantification of BrdU+Sox10+ cells, Ki67+PDGFRα+ cells on P3, and CC1+Sox10+ cells on P21. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction or unpaired Student’s t test. n.s. (not significant), P > 0.05; **P < 0.01.

    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Techniques: Expressing, Staining, Electron Microscopy, Immunofluorescence, Control, MANN-WHITNEY

    Fig. 8. Leukodystrophy-related RNF220 mutation knock-in mice show impaired regulation of learning and memory behaviors and of Olig proteins. (A to C) Behavioral tests of novel object recognition (A), three-chamber sociability, and social novelty [(B) and (C)], for P60 RNF220-RR (n = 15), RNF220-RQ (n = 15), and RNF220QQ (n = 15) mice. (A) Bar graphs (mean ± SD) show the percentage of spending time close to new object and logarithm of discrimination index. (B) Bar graphs (mean ± SD) show the percentage of spending time close to inanimate ball and animated stranger mouse and logarithm of social ratio. (C) Bar graphs (mean ± SD) show the percentage of spending time close to new and familiar animated strangers and logarithm of social ratio. (D) Ubiquitination analyses of polyubiquitinated Olig1 and Olig2 in the forebrains of P7 RNF220-RR (n = 3) and RNF220-QQ (n = 3) mice, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls. (E) WB analyses of endogenous protein expression of Olig1 and Olig2 in OPC and OL cells, isolated as in fig. S1A, from the brains of P7 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220QQ (n = 3) mice, and bar graphs (mean ± SD) show normalized levels against respective protein expression in the WT controls. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction or unpaired Student’s t test. n.s. (not significant), P > 0.05; **P < 0.01.

    Journal: Science advances

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.

    doi: 10.1126/sciadv.adk3931

    Figure Lengend Snippet: Fig. 8. Leukodystrophy-related RNF220 mutation knock-in mice show impaired regulation of learning and memory behaviors and of Olig proteins. (A to C) Behavioral tests of novel object recognition (A), three-chamber sociability, and social novelty [(B) and (C)], for P60 RNF220-RR (n = 15), RNF220-RQ (n = 15), and RNF220QQ (n = 15) mice. (A) Bar graphs (mean ± SD) show the percentage of spending time close to new object and logarithm of discrimination index. (B) Bar graphs (mean ± SD) show the percentage of spending time close to inanimate ball and animated stranger mouse and logarithm of social ratio. (C) Bar graphs (mean ± SD) show the percentage of spending time close to new and familiar animated strangers and logarithm of social ratio. (D) Ubiquitination analyses of polyubiquitinated Olig1 and Olig2 in the forebrains of P7 RNF220-RR (n = 3) and RNF220-QQ (n = 3) mice, and bar graphs (mean ± SD) show normalized polyubiquitination levels against respective expression in the WT controls. (E) WB analyses of endogenous protein expression of Olig1 and Olig2 in OPC and OL cells, isolated as in fig. S1A, from the brains of P7 RNF220-RR (n = 3), RNF220-RQ (n = 3), and RNF220QQ (n = 3) mice, and bar graphs (mean ± SD) show normalized levels against respective protein expression in the WT controls. Statistical analyses are compared to respective control with Mann-Whitney U test with Bonferroni correction or unpaired Student’s t test. n.s. (not significant), P > 0.05; **P < 0.01.

    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Techniques: Mutagenesis, Knock-In, Ubiquitin Proteomics, Expressing, Isolation, Control, MANN-WHITNEY